Phenyl Methyl Sulfonyl Fluoride as Protease Inhibitor – Essay Example
The paper "Phenyl Methyl Sulfonyl Fluoride as Protease Inhibitor" is an outstanding example of a biology essay. The process of protein purification and cell fractionation requires inhibition of protease activity. To this end, Phenyl Methyl Sulfonyl Fluoride (PMSF) is widely known as an irreversible inhibitor in relation to serine proteases like thrombin, trypsin and chymotrypsin (Rosenberg 122). In relation to protein purification, the enzymes that adhere firmly on peptide bonds, otherwise known as proteases, are potentially destructive contaminants that are unwanted and need to be removed or inactivated. Consequently, protease inhibitors such as PMSF are examples of protease inhibitors that inhibit proteolytic enzymes. Phenyl methyl sulfonyl fluoride is a second-generation protease inhibitor created as a replacement of diisopropyl fluorophosphates (DFP) (Scopes 322). DFP was an extremely dangerous and volatile serine protease inhibitor since it attacked serine hydrolase. Evidently, serine hydrolase plays a vital role in nerve conduction, human acetyl cholrinase. On the other hand, phenylmethylsulfonyl fluoride is not an active inhibitor of acetyl cholirinase. Consequently, it does not exhibit a volatile reaction due to an enzyme chemist attack on proteolysis. To this end, when PMSF is added directly to solutions composed of serine proteases, inhibition occurs whereby the enzymes are irreversibly inactivated. However, during isolation and protein purification, the sole use of PMSF may not be sufficient to protect proteins from degradation. This is normally evident when proteins are isolated tissues with high protease activity such as spleen and liver. Hence additional PMSF may be required or the choice of cocktail inhibitors such as EDTA, Leupeptin, PMSF and pepstadin.